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mouse pbabe retroviruses  (Addgene inc)


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    Addgene inc mouse pbabe retroviruses
    Mouse Pbabe Retroviruses, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    mouse pbabe retroviruses - by Bioz Stars, 2026-02
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    mouse pbabe retroviruses  (Addgene inc)
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    Mouse Pbabe Retroviruses, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    ha hif1alpha p402a p564a pbabe puro  (Addgene inc)
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.

    Journal: iScience

    Article Title: NRF2 regulates lipid droplet dynamics to prevent lipotoxicity

    doi: 10.1016/j.isci.2025.112925

    Figure Lengend Snippet: The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.

    Article Snippet: Overexpression plasmid pBabe-Puro (addgene #1764) was cut by multiple restriction enzymes, generating incompatible ends.

    Techniques: Expressing, Control, One-tailed Test, Over Expression